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In Vitro and in Vivo Association of FOG-1 and TACC3 —To examine further the specificity of the interaction between FOG-1 and TACC3, in vitro interactions were assessed by GST pull-down experiments. As shown in Fig. 2 a , in vitro expressed [ 35 S]met...



Interaction of endogenous FOG-1 and TACC3 was also examined in mammalian cells. For this purpose, a stable cell line derived from MEL cells that express an HA-tagged form of FOG-1 (or HA alone for control cells) to a level similar to that seen for th...



FOG-1 Changes Its Intracellular Localization in the Presence of TACC3 —FOG-1 and GATA-1 interact in the nucleus to control gene expression. However, TACC3 localizes in the cytoplasm and perinuclear region in interphase cells and associates with the c...







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