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     Phosphate buffered saline (PBS)
     Trypsin, cold sterilized in a 125 ml sterile erlenmeyer containing a magnetic stirring bar
     Minimum Essential Medium
     Fetal Calf Serum
     Clinical centrifuge with sterile capped centrifuge tubes
     Culture flasks
     Inverted phase contrast microscope (Optional) 

Procedure 8 

   1.Candle an 8 day old egg to ensure that it is alive. This is easily accomplished by holding the egg in front of a bright light source; the embryo can
     be seen as a shadow. Circle the embryo with a pencil. 

   2.Place the egg in a beaker with the blunt end up, and wash the top with a mild detergent, followed by swabbing with ethanol. 

   3.Carefully puncture the top of the egg with the point of a pair of sterile scissors and cut away a circle of shell, thus exposing the underlying
     membrane (the chorioallantois). 

   4.With a second pair of sterile scissors, carefully cut away and remove the chorioallantoic membrane, exposing the embryo. 

   5.Identify and carefully remove the embryo by the neck, using a sterile metal hook or a bent glass rod, and place the embryo in a 100mm petri dish