Bio-MUST-Apps-OmpaPa
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bin/ompa-pa.pl view on Meta::CPAN
#!/usr/bin/env perl
# PODNAME: ompa-pa.pl
# ABSTRACT: Extract seqs from BLAST/HMMER interactively or in batch mode
# CONTRIBUTOR: Amandine BERTRAND <amandine.bertrand@doct.uliege.be>
use Modern::Perl '2011';
use autodie;
use Getopt::Euclid qw(:vars);
use Smart::Comments;
use IO::Prompter [
-verbatim,
-style => 'blue strong dark',
-must => { 'be a string' => qr{\S+}xms }
];
use Bio::MUST::Core;
use aliased 'Bio::MUST::Core::Ali::Stash';
use aliased 'Bio::MUST::Core::Taxonomy';
use aliased 'Bio::MUST::Core::Taxonomy::ColorScheme';
use aliased 'Bio::MUST::Apps::OmpaPa::Blast';
use aliased 'Bio::MUST::Apps::OmpaPa::Hmmer';
# TODO: re-implement BLAST parsing and nr extraction ; done?
# setup OmpaPa sub-class based on report type
my $class = $ARGV_report_type eq 'blastxml' ? Blast : Hmmer;
my $scheme;
if ($ARGV_taxdir) {
my $tax = Taxonomy->new_from_cache( tax_dir => $ARGV_taxdir );
$scheme = $ARGV_colorize ? $tax->load_color_scheme($ARGV_colorize)
: ColorScheme->new(
tax => $tax,
names => [ qw(Archaea Bacteria Eukaryota Viruses) ],
colors => [ qw( blue green red orange) ],
)
;
}
# build args hash for Bio::MUST::Apps::OmpaPa::XXX constructor
# TODO: update attribute names
my %args;
$args{database} = $ARGV_database;
$args{extract_seqs} = 1 if $ARGV_extract_seqs;
$args{extract_taxs} = 1 if $ARGV_extract_tax;
$args{restore_last_param} = 1 if $ARGV_restore_last_params;
$args{nb_org} = $ARGV_max_copy;
$args{align} = $ARGV_min_cov;
# process infiles
for my $infile (@ARGV_infiles) {
### Processing: $infile
# read and parse report
my %new_args = (%args, file => $infile);
$new_args{scheme} = $scheme if $scheme;
$new_args{parameters} = $ARGV_restore_params_from
if $ARGV_restore_params_from;
my $oum = $class->new(%new_args);
say '[' . $oum->count_hits . ' hits processed] ';
if ($ARGV_print_plots) {
### Printing graphical selection with all possible colorations...
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