BioPerl
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Avg. full length: 747.2, trimmed (qual > -1): 684.2
Avg. quality: 48.3 per base
No. confirmed reads: 2
Avg. length: 748.5, confirmed: 671.0, str. confirmed: 670.0, trimmed: 703.0
Preliminary clone size estimate: 671 bp, depth of coverage: 2.0
Depth histogram (max_depth, #reads, cum #reads):
1 2 2
0 2 4
Forward confirmed bases: 0
Substitutions by nucleotide:
A C G T N X Z Total
A 0 0 0 0 0 0 0 0
C 0 0 0 0 0 0 0 0
G 0 0 0 0 0 0 0 0
T 0 0 0 0 0 0 0 0
N 0 0 0 0 0 0 0 0
X 0 0 0 0 0 0 0 0
Z 0 0 0 0 0 0 0 0
Substitutions by quality:
0 2 3 4 5 Total
0 0 0 0 0 0 0
2 0 0 0 0 0 0
3 0 0 0 0 0 0
4 0 0 0 0 0 0
5 0 0 0 0 0 0
Histogram of spacings between adjacent indel pairs:
Reverse confirmed bases: 1408
Subs: 46 (3.27%), dels: 0 (0.00%), inserts: 0 (0.00%)
Substitutions by nucleotide:
A C G T N X Z Total
A 0 1 8 0 0 0 0 9
C 1 0 18 8 0 0 0 27
G 2 2 0 1 0 0 0 5
T 2 2 1 0 0 0 0 5
N 0 0 0 0 0 0 0 0
X 0 0 0 0 0 0 0 0
Z 0 0 0 0 0 0 0 0
Substitutions by quality:
0 2 3 4 5 Total
0 0 1 7 7 8 23
2 1 0 0 0 0 1
3 7 0 0 0 0 7
4 7 0 0 0 0 7
5 8 0 0 0 0 8
Histogram of spacings between adjacent indel pairs:
Blocked reads:
ML4924F 11 600 left
ML4924R 56 753 left
2 blocked reads: 2 left only, 0 right only, 0 both.
0 reads (not shown) lack a high-quality segment.
0 perfect duplicates
2 isolated singlets (having no non-vector match to any other read):
Read Length (# trimmed non-X bases)
ML4922R 753 (668)
ML4947F 739 (658)
Contig 1. 2 reads; 708 bp (untrimmed), 704 (trimmed). Isolated contig.
-5 728 ML4924F 628 ( 0) 3.27 0.00 0.00 9 ( 9) 21 ( 54)
C 1 763 ML4924R 703 ( 0) 0.00 0.00 0.00 0 ( 3) 55 ( 89)
Overall discrep rates (%): 1.63 0.00 0.00
Contig quality (quality, n_residues, %, cum, cum %, cum expected errs):
90 62 8.8 62 8.8 0.00
89 7 1.0 69 9.7 0.00
88 11 1.6 80 11.3 0.00
87 7 1.0 87 12.3 0.00
86 19 2.7 106 15.0 0.00
85 14 2.0 120 16.9 0.00
84 4 0.6 124 17.5 0.00
83 8 1.1 132 18.6 0.00
82 3 0.4 135 19.1 0.00
81 8 1.1 143 20.2 0.00
80 22 3.1 165 23.3 0.00
79 4 0.6 169 23.9 0.00
78 17 2.4 186 26.3 0.00
77 16 2.3 202 28.5 0.00
76 10 1.4 212 29.9 0.00
75 29 4.1 241 34.0 0.00
74 12 1.7 253 35.7 0.00
73 12 1.7 265 37.4 0.00
72 22 3.1 287 40.5 0.00
71 22 3.1 309 43.6 0.00
70 9 1.3 318 44.9 0.00
69 14 2.0 332 46.9 0.00
68 3 0.4 335 47.3 0.00
67 18 2.5 353 49.9 0.00
66 9 1.3 362 51.1 0.00
65 4 0.6 366 51.7 0.00
64 23 3.2 389 54.9 0.00
63 6 0.8 395 55.8 0.00
62 12 1.7 407 57.5 0.00
61 15 2.1 422 59.6 0.00
60 29 4.1 451 63.7 0.00
59 11 1.6 462 65.3 0.00
58 34 4.8 496 70.1 0.00
57 7 1.0 503 71.0 0.00
56 14 2.0 517 73.0 0.00
55 6 0.8 523 73.9 0.00
54 8 1.1 531 75.0 0.00
53 9 1.3 540 76.3 0.00
52 6 0.8 546 77.1 0.00
51 48 6.8 594 83.9 0.00
50 8 1.1 602 85.0 0.00
49 6 0.8 608 85.9 0.00
48 3 0.4 611 86.3 0.00
47 5 0.7 616 87.0 0.00
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