Bio-Tools-Run-Alignment-TCoffee

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t/TCoffee.t  view on Meta::CPAN

use Test::More;
use File::Spec;

use Test::Exception;

use Bio::AlignIO;
use Bio::SeqIO;
use Bio::SimpleAlign;
use Bio::Tools::Run::Alignment::TCoffee;

sub test_input_file {
    return File::Spec->catfile('t', 'data', @_);
}

END { unlink qw(cysprot.dnd cysprot1a.dnd) }

END {
    unlink("t_coffee.log");
}

my @params;
my  $factory = Bio::Tools::Run::Alignment::TCoffee->new(@params);

isa_ok ($factory, 'Bio::Tools::Run::Alignment::TCoffee');

my $ktuple = 3;
$factory->ktuple($ktuple);

my $new_ktuple = $factory->ktuple();
is $new_ktuple, 3, "set factory parameter";

my $what_matrix = $factory->matrix();
like $what_matrix, qr/BLOSUM/i, "get factory parameter";

my $bequiet = 1;
$factory->quiet($bequiet);  # Suppress tcoffee messages to terminal

my $inputfilename = test_input_file('cysprot.fa');
my $aln;

my $version = $factory->_major_minor_version;

cmp_ok ($version, '>=', 1.22, "Code tested only on t_coffee versions > 1.22" );
$aln = $factory->align($inputfilename);
ok($aln);
is( $aln->num_sequences, 7);

my $str = Bio::SeqIO->new('-file' =>
                          test_input_file("cysprot.fa"),
                          '-format' => 'fasta');
my @seq_array =();

while ( my $seq = $str->next_seq() ) {
    push (@seq_array, $seq) ;
}

my $seq_array_ref = \@seq_array;

$aln = $factory->align($seq_array_ref);
is $aln->num_sequences, 7;
my $s1_perid = $aln->average_percentage_identity;

my $profile1 = test_input_file("cysprot1a.msf");
my $profile2 = test_input_file("cysprot1b.msf");

# convert any warnings about program to an actual exception
$factory->verbose(2);
lives_ok {$aln = $factory->profile_align($profile1,$profile2)};

ok(! $@, "no T-COFFEE errors");
is $aln->num_sequences, 7;

my $str1 = Bio::AlignIO->new(-file=> test_input_file("cysprot1a.msf"));
my $aln1 = $str1->next_aln();
is $aln1->num_sequences, 3;

my $str2 = Bio::AlignIO->new(-file=> test_input_file("cysprot1b.msf"));
my $aln2 = $str2->next_aln();
is $aln2->num_sequences, 4;

$aln = $factory->profile_align($aln1,$aln2);
is $aln->num_sequences, 7;

$str1 = Bio::AlignIO->new(-file=> test_input_file("cysprot1a.msf"));
$aln1 = $str1->next_aln();
$str2 = Bio::SeqIO->new(-file=> test_input_file("cysprot1b.fa"));
my $seq = $str2->next_seq();

is $aln1->num_sequences, 3;
is( int($aln1->average_percentage_identity), 39);
$aln = $factory->profile_align($aln1,$seq);
is( $aln->num_sequences, 4);
if( $version <= 1.22 ) {
    cmp_ok( $aln->overall_percentage_identity, '>', 18);
    is( int($aln->average_percentage_identity), 44);
} else {
    my $overall = int($aln->overall_percentage_identity);
    ok( $overall >=21 && $overall <= 23, 'expect 21 >= val >= 23');
    my $avg = int($aln->average_percentage_identity);
    ok( $avg == 47 || $avg ==48, 'expect 47 or 48');
}

# test new 'run' generic running of factory

$aln = $factory->run('-type' => 'align',
                     '-seq'  => test_input_file("cysprot.fa"));
is ($aln->num_sequences, 7, 'simple generic run');
is ($aln->percentage_identity,$s1_perid); #calculated before

lives_ok{ $aln = $factory->run('-type' => 'profile',
                               '-profile' => $aln1,
                               '-seq'  => test_input_file("cysprot1b.fa"))} ;

ok(! $@, "no T-COFFEE errors");

is( $aln->num_sequences, 7);
if( $version <= 1.22 ) {
    cmp_ok( $aln->overall_percentage_identity, '>', 18);
    is( int($aln->average_percentage_identity), 44);
} else {
    my $overall = int $aln->overall_percentage_identity;
    ok($overall == 14 || $overall == 13, 'expect 13 or 14');
    my $avg = int($aln->average_percentage_identity);
    ok($avg == 41 || $avg == 42, 'expect 41 or 42');
}

done_testing();

END {
    # warnings are already given above, no need to keep report
    if (-e 'error_report.T-COFFEE') {
        unlink('error_report.T-COFFEE');
    }
    if (my @dnds = glob('*.dnd')) {
        for my $file (@dnds) {
            unlink($file)
        }
    }
}



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