Bio-GeneDesign
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{
'buffers' => {
'NEB3' => '25',
'NEB1' => '25',
'Other' => '',
'NEB2' => '50',
'NEB4' => '100'
},
'palindromy' => 'nonpal',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0024535',
'score' => '0.0528',
'recseq' => 'CASTGNN',
'methcpg' => 'indifferent',
'methdam' => 'indifferent',
'length' => 7,
'temp' => '65',
'regex' => [
qr/CA[CGS]TG[ABCDGHKMNRSTVWY][ABCDGHKMNRSTVWY]/ix,
qr/[ABCDGHKMNRSTVWY][ABCDGHKMNRSTVWY]CA[CGS]TG/ix
],
'methdcm' => 'indifferent',
'type' => '3\'',
'_root_verbose' => 0,
'id' => 'TspRI',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'cutseq' => 'CASTGNN^',
'outside_cut' => 7,
'inside_cut' => 0,
'exclude' => []
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'PspOMI' => bless(
{
'buffers' => {
'NEB3' => '10',
'NEB1' => '25',
'Other' => '',
'NEB2' => '25',
'NEB4' => '100'
},
'recseq' => 'GGGCCC',
'methdam' => 'indifferent',
'id' => 'PspOMI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs',
'I' => 'SibEnzyme',
'V' => 'Vivantis'
},
'aggress' => '0.0002659',
'score' => '0.02826',
'length' => 6,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/GGGCCC/ix ],
'methdcm' => 'blocked',
'outside_cut' => 5,
'inside_cut' => 1,
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'G^GGCCC',
'exclude' => ['ApaI', 'BaeGI', 'BanII', 'Bsp1286I', 'CviKI', 'HaeIII', 'NlaIV', 'PhoI', 'Sau96I', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BsrI' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '0',
'Other' => '',
'NEB2' => '50',
'NEB4' => '10'
},
'recseq' => 'ACTGG',
'methdam' => 'indifferent',
'id' => 'BsrI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0016710',
'score' => '0.0464',
'length' => 5,
'methcpg' => 'indifferent',
'temp' => '65',
'regex' => [ qr/ACTGG/ix, qr/CCAGT/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'ACTGG(1/-1)',
'outside_cut' => 1,
'inside_cut' => -1,
'exclude' => ['BmrI']
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'TaqI' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '50',
'Other' => '',
'NEB2' => '75',
'NEB4' => '100'
},
'recseq' => 'TCGA',
'methdam' => 'blocked',
'staract' => 1,
'id' => 'TaqI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'S' => 'Sigma Aldrich',
'F' => 'Thermo Scientific Fermentas',
'N' => 'New England Biolabs',
'K' => 'Takara',
'Y' => 'CinnaGen',
'V' => 'Vivantis',
'Q' => 'Molecular Biology Resources',
'M' => 'Roche Applied Science',
'C' => 'Minotech',
'J' => 'Nippon Gene Co.',
'O' => 'Toyobo Technologies',
'X' => 'EURx',
'B' => 'Invitrogen',
'I' => 'SibEnzyme',
'R' => 'Promega',
'U' => 'Bangalore Genei'
},
'aggress' => '0.0024947',
'score' => '0.0106',
'length' => 4,
'methcpg' => 'indifferent',
'temp' => '65',
'regex' => [ qr/TCGA/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'T^CGA',
'outside_cut' => 3,
'inside_cut' => 1,
'exclude' => [ 'AsuII', 'BstBI', 'ClaI','PaeR7I', 'PspXI','SalI', 'XhoI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AcuI' => bless(
{
'buffers' => {
'NEB3' => '50',
'NEB1' => '50',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'CTGAAG',
'methdam' => 'indifferent',
'id' => 'AcuI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs',
'I' => 'SibEnzyme'
},
'aggress' => '0.0008247',
'score' => '0.1546',
'length' => 6,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/CTGAAG/ix, qr/CTTCAG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'CTGAAG(16/14)',
'outside_cut' => 16,
'inside_cut' => 14,
'exclude' => []
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AccI' => bless(
{
'buffers' => {
'NEB3' => '10',
'NEB1' => '50',
'Other' => '',
'NEB2' => '50',
'NEB4' => '100'
},
'recseq' => 'GTMKAC',
'methdam' => 'indifferent',
'id' => 'AccI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'S' => 'Sigma Aldrich',
'O' => 'Toyobo Technologies',
'J' => 'Nippon Gene Co.',
'N' => 'New England Biolabs',
'X' => 'EURx',
'K' => 'Takara',
'B' => 'Invitrogen',
'M' => 'Roche Applied Science',
'R' => 'Promega',
'U' => 'Bangalore Genei'
},
'aggress' => '0.0001856',
'score' => '0.0528',
'length' => 6,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/GT[ACM][GKT]AC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'GT^MKAC',
'outside_cut' => 4,
'inside_cut' => 2,
'exclude' => ['BstZ17I', 'Hpy166II', 'SalI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AarI' => bless(
{
'buffers' => {
'NEB3' => '',
'NEB1' => '',
'Other' => '100',
'NEB2' => '',
'NEB4' => ''
},
'recseq' => 'CACCTGC',
'methdam' => 'indifferent',
'id' => 'AarI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'F' => 'Thermo Scientific Fermentas'
},
'aggress' => '0.0002474',
'score' => '2.1600',
'length' => 7,
'methcpg' => 'inhibited',
'temp' => '37',
'regex' => [ qr/CACCTGC/ix, qr/GCAGGTG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'CACCTGC(4/8)',
'outside_cut' => 8,
'inside_cut' => 4,
'exclude' => ['BfuAI', 'BspMI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AciI' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '25',
'Other' => '',
'NEB2' => '50',
'NEB4' => '50'
},
'recseq' => 'CCGC',
'methdam' => 'indifferent',
'id' => 'AciI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0106387',
'score' => '0.244',
'length' => 4,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/CCGC/ix, qr/GCGG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'CCGC(-3/-1)',
'outside_cut' => -1,
'inside_cut' => -3,
'exclude' => [ 'BsrBI', 'EciI','FauI', 'NotI', 'SacII', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AhdI' => bless(
{
'buffers' => {
'NEB3' => '0',
'NEB1' => '25',
'Other' => '',
'NEB2' => '75',
'NEB4' => '100'
},
'recseq' => 'GACNNNNNGTC',
'methdam' => 'indifferent',
'id' => 'AhdI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0001856',
'score' => '0.066',
'length' => 11,
'methcpg' => 'inhibited',
'temp' => '37',
'regex' => [
qr/GAC[ABCDGHKMNRSTVWY][ABCDGHKMNRSTVWY][ABCDGHKMNRSTVWY][ABCDGHKMNRSTVWY][ABCDGHKMNRSTVWY]GTC/ix
],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'GACNNN^NNGTC',
'onebpoverhang' => 1,
'outside_cut' => 6,
'inside_cut' => 5,
'exclude' => []
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BsmI' => bless(
{
'buffers' => {
'NEB3' => '75',
'NEB1' => '75',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'GAATGC',
'methdam' => 'indifferent',
'id' => 'BsmI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'S' => 'Sigma Aldrich',
'O' => 'Toyobo Technologies',
'J' => 'Nippon Gene Co.',
'M' => 'Roche Applied Science',
'N' => 'New England Biolabs'
},
'aggress' => '0.0009484',
'score' => '0.0976',
'length' => 6,
'methcpg' => 'indifferent',
'temp' => '65',
'regex' => [ qr/GAATGC/ix, qr/GCATTC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'GAATGC(1/-1)',
'outside_cut' => 1,
'inside_cut' => -1,
'exclude' => []
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'MscI' => bless(
{
'buffers' => {
'NEB3' => '75',
'NEB1' => '75',
'Other' => '',
'NEB2' => '75',
'NEB4' => '100'
},
'recseq' => 'TGGCCA',
'methdam' => 'indifferent',
'id' => 'MscI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'unknown',
'vendors' => {
'O' => 'Toyobo Technologies',
'N' => 'New England Biolabs',
'B' => 'Invitrogen'
},
'aggress' => '0.0003711',
'score' => '0.2016',
'length' => 6,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/TGGCCA/ix ],
'methdcm' => 'blocked',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => 'b',
'cutseq' => 'TGG^CCA',
'exclude' => ['CviKI', 'EaeI', 'HaeIII', 'PhoI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BbvCI' => bless(
{
'buffers' => {
'NEB3' => '10',
'NEB1' => '50',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'CCTCAGC',
'methdam' => 'indifferent',
'id' => 'BbvCI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0001443',
'score' => '0.464',
'length' => 7,
'methcpg' => 'inhibited',
'temp' => '37',
'regex' => [ qr/CCTCAGC/ix, qr/GCTGAGG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'CCTCAGC(-5/-2)',
'outside_cut' => -2,
'inside_cut' => -5,
'exclude' => [ 'Bpu10I', 'BseMII', 'BspCNI', 'DdeI', 'MnlI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'RsaI' => bless(
{
'buffers' => {
'NEB3' => '50',
'NEB1' => '100',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'GTAC',
'methdam' => 'indifferent',
'id' => 'RsaI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'unknown',
'vendors' => {
'S' => 'Sigma Aldrich',
'F' => 'Thermo Scientific Fermentas',
'O' => 'Toyobo Technologies',
'J' => 'Nippon Gene Co.',
'X' => 'EURx',
'N' => 'New England Biolabs',
'Y' => 'CinnaGen',
'B' => 'Invitrogen',
'V' => 'Vivantis',
'Q' => 'Molecular Biology Resources',
'M' => 'Roche Applied Science',
'C' => 'Minotech',
'R' => 'Promega',
'I' => 'SibEnzyme'
},
'aggress' => '0.0023298',
'score' => '0.0424',
'length' => 4,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/GTAC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => 'b',
'cutseq' => 'GT^AC',
'exclude' => ['Acc65I', 'BsiWI', 'BsrGI', 'CviQI', 'KpnI', 'ScaI', 'TatI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AatII' => bless(
{
'buffers' => {
'NEB3' => '50',
'NEB1' => '0',
'Other' => '',
'NEB2' => '50',
'NEB4' => '100'
},
'recseq' => 'GACGTC',
'methdam' => 'indifferent',
'id' => 'AatII',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'F' => 'Thermo Scientific Fermentas',
'O' => 'Toyobo Technologies',
'M' => 'Roche Applied Science',
'N' => 'New England Biolabs',
'K' => 'Takara',
'R' => 'Promega',
'V' => 'Vivantis'
},
'aggress' => '0.0002062',
'score' => '0.0848',
'length' => 6,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/GACGTC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'GACGT^C',
'outside_cut' => 5,
'inside_cut' => 1,
'exclude' => ['BsaHI', 'HpyCH4IV','MaeII', 'TaiI', 'ZraI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BtrI' => bless(
{
'buffers' => {
'NEB3' => '',
'NEB1' => '',
'Other' => 'O',
'NEB2' => '',
'NEB4' => ''
},
'recseq' => 'CACGTC',
'methdam' => 'indifferent',
'id' => 'BtrI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'I' => 'SibEnzyme',
'V' => 'Vivantis'
},
'aggress' => '0.0003505',
'score' => '9.999',
'length' => 6,
'methcpg' => 'indifferent',
'temp' => '60',
'regex' => [ qr/CACGTC/ix, qr/GACGTG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => 'b',
'cutseq' => 'CACGTC(-3/-3)',
'exclude' => [ 'BmgBI', 'HpyCH4IV', 'MaeII', 'TaiI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BmgBI' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '0',
'Other' => '',
'NEB2' => '25',
'NEB4' => '10'
},
'recseq' => 'CACGTC',
'methdam' => 'indifferent',
'id' => 'BmgBI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0003505',
'score' => '0.232',
'length' => 6,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/CACGTC/ix, qr/GACGTG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => 'b',
'cutseq' => 'CACGTC(-3/-3)',
'exclude' => [ 'BtrI', 'HpyCH4IV', 'MaeII', 'TaiI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'PspXI' => bless(
{
'buffers' => {
'NEB3' => '10',
'NEB1' => '0',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'VCTCGAGB',
'methdam' => 'indifferent',
'id' => 'PspXI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs',
'I' => 'SibEnzyme'
},
'aggress' => '0.0000206',
'score' => '0.252',
'length' => 8,
'methcpg' => 'inhibited',
'temp' => '37',
'regex' => [ qr/[ACGMRSV]CTCGAG[BCGKSTY]/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'VC^TCGAGB',
'outside_cut' => 6,
'inside_cut' => 2,
'exclude' => [ 'AvaI', 'BsoBI', 'PaeR7I', 'SmlI', 'TaqI', 'XhoI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'PspGI' => bless(
{
'buffers' => {
'NEB3' => '50',
'NEB1' => '75',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0000501',
'score' => '0.0464',
'recseq' => 'CCWGG',
'methcpg' => 'indifferent',
'methdam' => 'indifferent',
'length' => 5,
'temp' => '75',
'regex' => [ qr/CC[ATW]GG/ix ],
'methdcm' => 'blocked',
'type' => '5\'',
'_root_verbose' => 0,
'id' => 'PspGI',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'cutseq' => '^CCWGG',
'outside_cut' => 5,
'inside_cut' => 0,
'exclude' => ['BssKI', 'BstNI', 'EcoRII', 'PasI', 'ScrFI', 'SexAI', 'StyD4I', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'MlyI' => bless(
{
'buffers' => {
'NEB3' => '25',
'NEB1' => '50',
'Other' => '',
'NEB2' => '50',
'NEB4' => '100'
},
'recseq' => 'GAGTC',
'methdam' => 'indifferent',
'id' => 'MlyI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0012577',
'score' => '0.0464',
'length' => 5,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/GAGTC/ix, qr/GACTC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => 'b',
'cutseq' => 'GAGTC(5/5)',
'exclude' => [ 'HinfI', 'PleI',]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BmtI' => bless(
{
'buffers' => {
'NEB3' => '25',
'NEB1' => '25',
'Other' => '',
'NEB2' => '100',
'NEB4' => '50'
},
'recseq' => 'GCTAGC',
'methdam' => 'indifferent',
'id' => 'BmtI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs',
'I' => 'SibEnzyme',
'V' => 'Vivantis'
},
'aggress' => '0.0000206',
'score' => '0.1546',
'length' => 6,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/GCTAGC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'GCTAG^C',
'outside_cut' => 5,
'inside_cut' => 1,
'exclude' => ['BfaI', 'Cac8I', 'MaeI', 'NheI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BsrBI' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '50',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'CCGCTC',
'methdam' => 'indifferent',
'id' => 'BsrBI',
'tempin' => '80',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0003505',
'score' => '0.0488',
'length' => 6,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/CCGCTC/ix, qr/GAGCGG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => 'b',
'cutseq' => 'CCGCTC(-3/-3)',
'exclude' => [ 'AciI' ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'AlwI' => bless(
{
'buffers' => {
'NEB3' => '10',
'NEB1' => '50',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'GGATC',
'methdam' => 'blocked',
'id' => 'AlwI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0011958',
'score' => '0.0976',
'length' => 5,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/GGATC/ix, qr/GATCC/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'GGATC(4/5)',
'onebpoverhang' => 1,
'outside_cut' => 5,
'inside_cut' => 4,
'exclude' => ['BamHI', 'BfuCI', 'DpnI', 'DpnII', 'Sau3AI',]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BtsCI' => bless(
{
'buffers' => {
'NEB3' => '50',
'NEB1' => '50',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0030927',
'score' => '0.0252',
'recseq' => 'GGATG',
'methcpg' => 'indifferent',
'methdam' => 'indifferent',
'length' => 5,
'temp' => '50',
'regex' => [ qr/GGATG/ix, qr/CATCC/ix ],
'methdcm' => 'indifferent',
'type' => '3\'',
'_root_verbose' => 0,
'id' => 'BtsCI',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'cutseq' => 'GGATG(2/0)',
'outside_cut' => 2,
'inside_cut' => 0,
'exclude' => ['FokI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
t/30-enzymes.t view on Meta::CPAN
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'NlaIII' => bless(
{
'buffers' => {
'NEB3' => '25',
'NEB1' => '25',
'Other' => '',
'NEB2' => '25',
'NEB4' => '100'
},
'recseq' => 'CATG',
'methdam' => 'indifferent',
'id' => 'NlaIII',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0040847',
'score' => '0.0976',
'length' => 4,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/CATG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '3\'',
'cutseq' => 'CATG^',
'outside_cut' => 4,
'inside_cut' => 0,
'exclude' => [ 'BspHI', 'CviAII', 'FatI', 'NcoI', 'NspI', 'PciI', 'SphI', ]
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'BseYI' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '10',
'Other' => '',
'NEB2' => '50',
'NEB4' => '50'
},
'recseq' => 'CCCAGC',
'methdam' => 'indifferent',
'id' => 'BseYI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pnon',
'vendors' => {
'N' => 'New England Biolabs'
},
'aggress' => '0.0006598',
'score' => '0.488',
'length' => 6,
'methcpg' => 'blocked',
'temp' => '37',
'regex' => [ qr/CCCAGC/ix, qr/GCTGGG/ix ],
'methdcm' => 'indifferent',
'class' => 'IIA',
'classex' => qr/\A \w+ \(([\-]*\d+) \/ ([\-]*\d+)\)\Z /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'CCCAGC(-5/-1)',
'outside_cut' => -1,
'inside_cut' => -5,
'exclude' => []
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'MseI' => bless(
{
'buffers' => {
'NEB3' => '75',
'NEB1' => '75',
'Other' => '',
'NEB2' => '100',
'NEB4' => '100'
},
'recseq' => 'TTAA',
'methdam' => 'indifferent',
'id' => 'MseI',
'tempin' => '65',
'timein' => '20',
'palindromy' => 'pal',
'vendors' => {
'N' => 'New England Biolabs',
'B' => 'Invitrogen'
},
'aggress' => '0.0040205',
'score' => '0.0976',
'length' => 4,
'methcpg' => 'indifferent',
'temp' => '37',
'regex' => [ qr/TTAA/ix ],
'methdcm' => 'indifferent',
'class' => 'IIP',
'classex' => qr/ ([A-Z]*) \^ ([A-Z]*) /x,
'_root_verbose' => 0,
'type' => '5\'',
'cutseq' => 'T^TAA',
'outside_cut' => 3,
'inside_cut' => 1,
'exclude' => ['AflII', 'AseI', 'DraI', 'HpaI', 'PacI', 'PmeI', 'SwaI']
},
'Bio::GeneDesign::RestrictionEnzyme'
),
'Bpu10I' => bless(
{
'buffers' => {
'NEB3' => '100',
'NEB1' => '10',
'Other' => '',
'NEB2' => '25',
'NEB4' => '25'
},
( run in 1.050 second using v1.01-cache-2.11-cpan-5a3173703d6 )