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scripts/Tutorial_pipeline03.pl  view on Meta::CPAN

The result of this tutorial can be viewed by navigating your browser
L<here|http://genome-euro.ucsc.edu/cgi-bin/hgTracks?hubUrl=http://nibiru.tbi.univie.ac.at/ViennaNGS/tutorial03/hg19_trackHub/trackHub/hub.txt&position=chr15>

This tutorial is based on the track_hub_constructor.pl script and the
output from Tutorial02_pipeline.pl.  While the option descriptions
here are specific for the results from Tutorial02 the
trackhub_hub_constructor.pl can be applied in the same manner to other
datasets. The example call uses the bigwig and bedfiles available from
our server.  Example call:

  Tutorial_pipeline03.pl -o /home/user/public_html/hg19_trackHub -u
http://www.mydomain.com/ucsc/hg19_trackHub -b
http://www.mydomain.com/ucsc/data/hg19_highlyexpressed.pos.bb#http://www.mydomain.com/ucsc/data/hg19_highlyexpressed.neg.bb
                                                             #-w
                                                             #http://www.mydomain.com/ucsc/data/hg19_highlyexpressed.pos.bw,http://www.mydomain.com/ucsc/data/hg19_highlyexpressed.neg.bw

=head2 PREREQUITES

To run this tutorial on your machine you will need a full installation
of the L<Bio::ViennaNGS> distribution. Input files can be downloaded
L<here|http://nibiru.tbi.univie.ac.at/ViennaNGS>):

=over

=item F<hg19_highlyexpressed.pos.bb>

=item F<hg19_highlyexpressed.neg.bb>

=item F<hg19_highlyexpressed.pos.bw>

=item F<hg19_highlyexpressed.neg.bw>

=back

For the UCSC genome browser to be able to visualize our trackhub it
needs to be accessible via URL. This is the base URL you need to
provide as commandline argument.

=head2 DISCLAIMER

The resulting trackhub can only be read by the UCSC genome browser if
it is accessible via URL.  If you have no webspace for testing
available you can use example output available from our webserver
L<here|http://nibiru.tbi.univie.ac.at/ViennaNGS>.


=head1 PIPELINE

=head3 Create UCSC Genome Browser Trackhub

  print "Constructing UCSC genome browser trackhub ...\n";
  
  $track_hub_return_value = make_track_hub($genome_identifier,$dest,$base_URL,$big_bed_urls,$big_wig_urls,$lf);
  
  print "Loading the trackhub into the UCSC genome browser...\n\n";
  
  print "1. Point your browser to: http://genome.ucsc.edu/index.html\n\n";
  
  print "2. Select Genome Browser from the left menu\n\n";
  
  print "3. You are now redirected to nearest mirror of the genome browser.\n    Select \"My Data\" from the top menu and then \"Track Hubs\" in the popup list.\n\n";
  
  print "4. The \"Track Data Hubs\" page is displayed. In the register \"My Hubs\" it is possible to add the newly created track hub. Paste the URL to hub.txt into the URL field and click \"Add Hub\" (e.g. http://nibiru.tbi.univie.ac.at/ViennaNGS/tut...
  
  print "5. The track hub now loads into the hg19 public hub. \n\n";
  
  print "6. Enter e.g. chr15 in the position field and hit go\n\n";
  
  print "7. You should now see 2 annotation tracks and a bigwig multi-track plotted in red and green\n\n";
  
  print "DONE\n";

=cut

print "Constructing UCSC genome browser trackhub ...\n";

$track_hub_return_value = make_track_hub($genome_identifier,$dest,$base_URL,$big_bed_urls,$big_wig_urls,$lf);

print "Loading the trackhub into the UCSC genome browser...\n\n";

print "1. Point your browser to: http://genome.ucsc.edu/index.html\n\n";

print "2. Select Genome Browser from the left menu\n\n";

print "3. You are now redirected to nearest mirror of the genome browser.\n    Select \"My Data\" from the top menu and then \"Track Hubs\" in the popup list.\n\n";

print "4. The \"Track Data Hubs\" page is displayed. In the register \"My Hubs\" it is possible to add the newly created track hub. Paste the URL to hub.txt into the URL field and click \"Add Hub\" (e.g. http://nibiru.tbi.univie.ac.at/ViennaNGS/tutor...

print "5. The track hub now loads into the hg19 public hub. \n\n";

print "6. Enter e.g. chr15 in the position field and hit go\n\n";

print "7. You should now see 2 annotation tracks and a bigwig multi-track plotted in red and green\n\n";

print "DONE\n";

=head1 COMMAND LINE OPTIONS

=cut

__END__


=over 4

=head1 OPTIONS

=over

=item B<--out -o>

Destination folder for the output Track Hub.

=item  B<--baseurl -u>

BaseURL used within the Track Hub. This URL will be included verbatim
in the resulting Track Hub. It is crucial that this URl is valid, else
the resulting Track Hub will be broken.

=item  B<--bigbeds -b>

URLs pointing to big bed files to be included in the trackhub. Multiple URLs are
separated by the character #. 

=item  B<--bigwigs -w>

URLs pointing to big wig files to be included in the trackhub. Multiple URLs are
separated by the character #. It is possible to create a multiwig container by
providing 2 URLs instead of one separated by comma character ,. E.g.
http://foo.com/bar.bw,http://foo.com/bar2.bw#http://foo.com/bar3.bw yields a multi
big wig container displaying bar as positive reads in green and bar2 as negative
3 red colored reads in the same track and additionally bar3 in an own track
colored blue.

=item B<--help -h>

Print short help

=item B<--man>

Prints the manual page and exits

=back

=head1 AUTHORS