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id: MI:0402
name: chromatin immunoprecipitation assay
def: "Chromatin immunoprecipitation (ChIP) is a powerful approach that allows one to define the interaction of factors with specific chromosomal sites in living cells. ChIP involves treating cells or tissue briefly with formaldehyde to crosslink prot...
subset: PSI-MI slim
synonym: "ch-ip" EXACT PSI-MI-short []
is_a: MI:0400 ! affinity technology

[Term]
id: MI:0403
name: colocalization
def: "Coincident occurrence of molecules in a given subcellular fraction observed with a low resolution methodology from which a physical interaction among those molecules cannot be inferred." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0190 ! interaction type

[Term]
id: MI:0404
name: comigration in non denaturing gel electrophoresis
def: "comigration by non denaturing gel electrophoresis." [PMID:14755292]
subset: PSI-MI slim
synonym: "comig non denat gel" EXACT PSI-MI-short []
is_a: MI:0807 ! comigration in gel electrophoresis

[Term]
id: MI:0405
name: competition binding
def: "Competitive binding experiments measure equilibrium binding of a single concentration of ligand at various concentrations of an unlabeled competitor. Analysis of these data gives the affinity of the receptor for the competitor." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0400 ! affinity technology

[Term]
id: MI:0406
name: deacetylase assay
def: "Measures the catalysis of the hydrolysis of an acetyl group or groups from a substrate molecule." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0415 ! enzymatic study

[Term]
id: MI:0407
name: direct interaction
def: "Interaction that is proven to involve only its interactors." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0915 ! physical association

[Term]
id: MI:0408
name: disulfide bond
def: "Covalent bond mediated by 2 sulfur atoms." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0195 ! covalent binding

[Term]
id: MI:0409
name: dna footprinting
def: "Experimental method used to identify the region of a nucleic acid involved in an interaction with a protein. One sample of a radiolabeled nucleic acid of known sequence is submitted to partial digestion. A second sample is incubated with its in...
subset: PSI-MI slim
is_obsolete: true

[Term]
id: MI:0410
name: electron tomography
def: "Electron tomography is a general method for three-dimensional (3D) reconstruction of single, transparent objects from a series of projection images (i.e. from a tilt series) recorded with a transmission electron microscope. It offers the opport...
subset: PSI-MI slim
synonym: "sem" EXACT PSI-MI-short []
is_a: MI:0040 ! electron microscopy

[Term]
id: MI:0411
name: enzyme linked immunosorbent assay
def: "Following non-covalent binding of a purified primary ligand to a solid phase, a blocking reagent is added to prevent any non-specific binding. A specific antigen is then allowed to bind to the primary ligand. Unbound antigen is removed by washi...
subset: PSI-MI slim
synonym: "ELISA" EXACT PSI-MI-alternate []
synonym: "elisa" EXACT PSI-MI-short []
is_a: MI:0421 ! identification by antibody
is_a: MI:0892 ! solid phase assay

[Term]
id: MI:0412
name: electrophoretic mobility supershift assay
def: "The EMSA supershift is a EMSA experiment carried out using a third lane loaded with the radiolabeled nucleic acid, a protein mixture and an antibody for a specific protein. If an extra retardation is observed, this is due to the formation of a ...
subset: PSI-MI slim
synonym: "emsa supershift" EXACT PSI-MI-short []
is_a: MI:0413 ! electrophoretic mobility shift assay

[Term]
id: MI:0413
name: electrophoretic mobility shift assay
def: "This method proves the interaction between a nucleic acid and a protein partner. On the same electrophoresis gel 1 lane is loaded with a radiolabeled nucleic acid of known sequence, a second lane is loaded with the same nucleic acid together wi...
subset: PSI-MI slim
synonym: "band shift" EXACT PSI-MI-alternate []
synonym: "emsa" EXACT PSI-MI-short []
synonym: "Gel retardation assay" EXACT PSI-MI-alternate []
is_a: MI:0892 ! solid phase assay

[Term]
id: MI:0414
name: enzymatic reaction
def: "terms aiming to represent biochemical reactions referring to their resulting product modifications." [PMID:14755292]
subset: PSI-MI slim
synonym: "Biochemical reaction" EXACT PSI-MI-alternate []
is_a: MI:0407 ! direct interaction

[Term]
id: MI:0415
name: enzymatic study
def: "Participants are enzyme or substrate in a biochemical reaction." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0401 ! biochemical

[Term]
id: MI:0416
name: fluorescence microscopy
def: "Fluorescent microscopy uses a high intensity light to illuminate the sample. This light excites fluorescence species in the sample, which then emit light of a longer wavelength. A fluorescent microscope also produces a magnified image of the sa...
subset: PSI-MI slim
synonym: "fluorescence imaging" EXACT PSI-MI-short []
is_a: MI:0428 ! imaging technique

[Term]
id: MI:0417
name: footprinting
def: "footprinting analysis is used to identify regions of molecules directly involved in binding other macromolecules and therefore protected from the effects of degradative enzymes." [PMID:14755292]
subset: PSI-MI slim