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subset: PSI-MI slim
synonym: "ENDOR" EXACT PSI-MI-alternate []
synonym: "endor" EXACT PSI-MI-short []
is_a: MI:0043 ! electron resonance

[Term]
id: MI:0042
name: electron paramagnetic resonance
def: "EPR (also called ESR, Electron Spin Resonance) spectroscopy is analogous to NMR, but is based on the excitation of unpaired electrons instead of nuclei. Unpaired (single) electrons are only found in radicals and some metal ions (paramagnetic sp...
subset: PSI-MI slim
synonym: "EPR" EXACT PSI-MI-alternate []
synonym: "epr" EXACT PSI-MI-short []
synonym: "ESR" EXACT PSI-MI-alternate []
is_a: MI:0043 ! electron resonance

[Term]
id: MI:0043
name: electron resonance
def: "A form of spectroscopy in which the absorption of microwave by a sample in a strong magnetic field is used to study atoms or molecules with unpaired electrons." [PMID:14755292]
subset: PSI-MI slim
is_a: MI:0013 ! biophysical
is_a: MI:0659 ! experimental feature detection

[Term]
id: MI:0045
name: experimental interaction detection
def: "Methods based on laboratory experiments to determine an interaction." [PMID:14755292]
subset: PSI-MI slim
synonym: "experimental interac" EXACT PSI-MI-short []
is_a: MI:0001 ! interaction detection method

[Term]
id: MI:0046
name: experimental knowledge based
def: "Predictive algorithms that rely on the information obtained by experimental results." [PMID:14755292]
subset: PSI-MI slim
synonym: "experimental info" EXACT PSI-MI-short []
is_a: MI:0063 ! interaction prediction

[Term]
id: MI:0047
name: far western blotting
def: "Proteins are fractionated by PAGE (SDS-polyacrylamide gel electrophoresis), transferred to a nitrocellulose membrane and tested for the ability to bind to a protein, a peptide, or any other ligand. Cell lysates can also be fractionated before g...
subset: PSI-MI slim
synonym: "Affinity blotting" EXACT PSI-MI-alternate []
is_a: MI:0892 ! solid phase assay

[Term]
id: MI:0048
name: filamentous phage display
def: "Filamentous phages (M13, f1, fd) have been extensively used to develop and implement the technology of phage display. Repertoires of relatively short peptides of random amino acid sequences or cDNA libraries have been constructed and searched s...
subset: PSI-MI slim
synonym: "filamentous phage" EXACT PSI-MI-short []
is_a: MI:0084 ! phage display

[Term]
id: MI:0049
name: filter binding
def: "A method in which separation depends upon the ability of one participant to bind to a filter or membrane which the other participants do not. Molecules interacting with the bound molecule will also be retain on the filter. For example, proteins...
subset: PSI-MI slim
synonym: "Filter overlay assay" EXACT PSI-MI-alternate []
is_a: MI:0892 ! solid phase assay

[Term]
id: MI:0050
name: flag tag coimmunoprecipitation
def: "The protein of interest is expressed as a fusion to the peptide DYKDDDDKV for which antibodies are commercially available. Sometimes multiple copies of the peptide are fused in tandem.\nOBSOLETE redundant term. Map to feature type: flag-tagged ...
subset: PSI-MI slim
synonym: "flag tag coip" EXACT PSI-MI-short []
is_obsolete: true

[Term]
id: MI:0051
name: fluorescence technology
def: "Techniques based upon the measurement of the emission of one or more photons by a molecule activated by the absorption of a quantum of electro-magnetic radiation. Typically the emission, which is characterised by a wavelength that is longer tha...
subset: PSI-MI slim
synonym: "fluorescence" EXACT PSI-MI-short []
is_a: MI:0013 ! biophysical

[Term]
id: MI:0052
name: fluorescence correlation spectroscopy
def: "FCS monitors the random motion of fluorescently labelled molecules inside a defined volume irradiated by a focused laser beam. These fluctuations provide information on the rate of diffusion or diffusion time of a particle and this is directly ...
subset: PSI-MI slim
synonym: "FCS" EXACT PSI-MI-alternate []
synonym: "fcs" EXACT PSI-MI-short []
is_a: MI:0051 ! fluorescence technology

[Term]
id: MI:0053
name: fluorescence polarization spectroscopy
def: "Because of the long lifetimes of excited fluorescent molecules (nanoseconds), fluorescence can be used to monitor the rotational motion of molecules, which occurs on this timescale. This is accomplished experimentally by excitation with plane-p...
subset: PSI-MI slim
synonym: "Fluorescence anisotropy" EXACT PSI-MI-alternate []
synonym: "FPS" EXACT PSI-MI-alternate []
synonym: "fps" EXACT PSI-MI-short []
is_a: MI:0051 ! fluorescence technology

[Term]
id: MI:0054
name: fluorescence-activated cell sorting
def: "Cells in suspension flow through a laser beam, the scattered light or emitted fluorescence is measured, filtered and converted to digital values. Cells can be sorted according to their properties. Using flow cytometry, any fluorescent or light ...
subset: PSI-MI slim
synonym: "FACS" EXACT PSI-MI-alternate []
synonym: "facs" EXACT PSI-MI-short []
synonym: "Flow cytometry" EXACT PSI-MI-alternate []
is_a: MI:0051 ! fluorescence technology

[Term]
id: MI:0055
name: fluorescent resonance energy transfer
def: "FRET is a quantum mechanical process involving the radiationless transfer of energy from a donor fluorophore to an appropriately positioned acceptor fluorophore. The fluorophores are genetically fused to the protein in analysis and cotransfecte...
subset: PSI-MI slim
synonym: "FRET" EXACT PSI-MI-alternate []
synonym: "fret" EXACT PSI-MI-short []
synonym: "FRET analysis" EXACT PSI-MI-alternate []
synonym: "RET" EXACT PSI-MI-alternate []
is_a: MI:0051 ! fluorescence technology

[Term]
id: MI:0056