BioPerl-Run
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lib/Bio/Tools/Run/Phylo/Phylip/ProtPars.pm view on Meta::CPAN
#Create the Tree
#using a threshold value of 30 and id name lengths limit of 30
#note to use id name length greater than the standard 10 in protpars,
# you will need to modify the protpars source code
$tree_factory = Bio::Tools::Run::Phylo::Phylip::ProtPars->
new(idlength=>30,threshold=>10,jumble=>"17,10",outgroup=>2);
$tree = $tree_factory->run($aln);
#Or one can pass in a file name containing a multiple alignment
#in phylip format:
$tree_factory =
Bio::Tools::Run::Phylo::Phylip::ProtPars->new(idlength=>30,threshold=>10);
$tree = $tree_factory->run("/usr/users/shawnh/COMPARA/prot.phy");
# To prevent PHYLIP from truncating sequence names:
# Step 1. Shelf the original names:
my ($aln_safe, $ref_name)= # $aln_safe has serial names
$aln->set_displayname_safe(); # $ref_name holds original names
# Step 2. Run ProtPars:
$tree = $protpars_factory->run($aln_safe); # Use $aln_safe instead of $aln
# Step 3. Retrieve orgininal OTU names:
use Bio::Tree::Tree;
my @nodes=$tree->get_nodes();
foreach my $nd (@nodes){
$nd->id($ref_name->{$nd->id_output}) if $nd->is_Leaf;
}
=head1 PARAMTERS FOR PROTPARS COMPUTATION
=head2 THRESHOLD
Title : THRESHOLD
Description : (optional)
This sets a threshold such that if the number of
steps counted in a character is higher than the
threshold, it will be taken to be the threshold
value rather than the actual number of steps. You
should use a positive real number greater than 1.
Please see the documetation from the phylip package
for more information.
=head2 OUTGROUP
Title : OUTGROUP
Description : (optional)
This specifies which species is to be used to root
the tree by having it become the outgroup. Input
values are integers specifying which species to use.
Defaults to 1
=head2 JUMBLE
Title : JUMBLE
Description : (optional)
This enables you to tell the program to use a random
number generator to choose the input order of
species. Input values is of the format:
seed,iterations eg 17,10 seed: an integer between 1
and 32767 and of the form 4n+1 which means that it
must give a remainder of 1 when divided by 4. Each
different seed leads to a different sequence of
addition of species. By simply changing the random
number seed and re-running programs one can look for
other, and better trees. iterations: For a value of
10, this will tell the program to try ten different
orders of species in constructing the trees, and the
results printed out will reflect this entire search
process (that is, the best trees found among all 10
runs will be printed out, not the best trees from
each individual run).
=head1 FEEDBACK
=head2 Mailing Lists
User feedback is an integral part of the evolution of this and other
Bioperl modules. Send your comments and suggestions preferably to one
of the Bioperl mailing lists. Your participation is much appreciated.
bioperl-l@bioperl.org - General discussion
http://bioperl.org/wiki/Mailing_lists - About the mailing lists
=head2 Support
Please direct usage questions or support issues to the mailing list:
I<bioperl-l@bioperl.org>
rather than to the module maintainer directly. Many experienced and
reponsive experts will be able look at the problem and quickly
address it. Please include a thorough description of the problem
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
http://redmine.open-bio.org/projects/bioperl/
=head1 AUTHOR - Shawn Hoon
Email shawnh@fugu-sg.org
=head1 CONTRIBUTORS
Email jason-AT-bioperl_DOT_org
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
package Bio::Tools::Run::Phylo::Phylip::ProtPars;
use vars qw($AUTOLOAD @ISA $PROGRAM $PROGRAMDIR $PROGRAMNAME
@PROTPARS_PARAMS @OTHER_SWITCHES
%OK_FIELD);
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