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lib/Bio/Roary/CommandLine/GeneAlignmentFromNucleotides.pm  view on Meta::CPAN

undef $VERSION;
package Bio::Roary::CommandLine::GeneAlignmentFromNucleotides;
$Bio::Roary::CommandLine::GeneAlignmentFromNucleotides::VERSION = '3.13.0';
# ABSTRACT: Take in a multifasta file of nucleotides, convert to proteins and align with PRANK


use Moose;
use Getopt::Long qw(GetOptionsFromArray);
use File::Copy;
use Bio::Roary::AnnotateGroups;
use Bio::Roary::External::Prank;
use Bio::Roary::Output::GroupsMultifastaProtein;
use Bio::Roary::SortFasta;
use Bio::Roary::External::Mafft;
extends 'Bio::Roary::CommandLine::Common';

has 'args'        => ( is => 'ro', isa => 'ArrayRef', required => 1 );
has 'script_name' => ( is => 'ro', isa => 'Str',      required => 1 );
has 'help'        => ( is => 'rw', isa => 'Bool',     default  => 0 );

has 'nucleotide_fasta_files' => ( is => 'rw', isa => 'ArrayRef' );
has '_error_message'         => ( is => 'rw', isa => 'Str' );
has 'verbose'                => ( is => 'rw', isa => 'Bool', default => 0 );
has 'mafft'                  => ( is => 'rw', isa => 'Bool', default => 0 );
has '_min_similarity'        => ( is => 'rw', isa => 'Num',  default  => 0.98 );

sub BUILD {
    my ($self) = @_;

    my ( $nucleotide_fasta_files, $help, $verbose,$mafft, );

    GetOptionsFromArray(
        $self->args,
        'v|verbose' => \$verbose,
        'n|mafft'   => \$mafft,
        'h|help'    => \$help,
    );

    if ( defined($verbose) ) {
        $self->verbose($verbose);
        $self->logger->level(10000);
    }
    $self->mafft($mafft) if (defined($mafft));
    $self->help($help) if ( defined($help) );
    if ( @{ $self->args } == 0 ) {
        $self->_error_message("Error: You need to provide at least 1 FASTA file");
    }

    for my $filename ( @{ $self->args } ) {
        if ( !-e $filename ) {
            $self->_error_message("Error: Cant access file $filename");
            last;
        }
    }
    $self->nucleotide_fasta_files( $self->args );
}

sub run {
    my ($self) = @_;

    ( !$self->help ) or die $self->usage_text;
    if ( defined( $self->_error_message ) ) {
        print $self->_error_message . "\n";
        die $self->usage_text;
    }

    for my $fasta_file ( @{ $self->nucleotide_fasta_files } ) {

        my $sort_fasta_before = Bio::Roary::SortFasta->new(
            input_filename         => $fasta_file,
            make_multiple_of_three => 1,
        );
        $sort_fasta_before->sort_fasta->replace_input_with_output_file;

        if ( $sort_fasta_before->sequences_unaligned == 1  || $sort_fasta_before->sequences_unaligned == 0 && $sort_fasta_before->similarity <= $self->_min_similarity) {

            if ( $self->mafft == 1 ) {