Bio-Graphics
view release on metacpan or search on metacpan
lib/Bio/Graphics/Glyph/vista_plot.pm view on Meta::CPAN
$v_range = 0;
$v_start = 255;
}
elsif ( $start_color eq 'white' ) { # end black
$s_range = 0;
$s_start = 0;
$v_range = -255;
$v_start = 255;
}
elsif ( $stop_color eq 'white' ) { # start black
$s_range = 0;
$s_start = 0;
$v_range = 255;
$v_start = 0;
}
elsif ( _isa_color($start_color) ) { # end black
$s_range = 255;
$s_start = 0;
$v_range = 255;
$v_start = 0;
}
elsif ( _isa_color($stop_color) ) { # start black
$s_range = -255;
$s_start = 255;
$v_range = -255;
$v_start = 255;
}
}
# store gradient info
$self->h_range($h_range);
$self->h_start($h_start);
$self->s_start($s_start);
$self->v_start($v_start);
$self->s_range($s_range);
$self->v_range($v_range);
# store score info
$self->peak_score_range($max - $min);
$self->min_peak_score($min);
$self->max_peak_score($max);
# store color extremes
my @low_rgb = $self->HSVtoRGB(@$hsv_start);
my @high_rgb = $self->HSVtoRGB(@$hsv_stop);
$self->low_hsv($hsv_start);
$self->high_rgb(\@high_rgb);
$self->low_rgb(\@low_rgb);
return 1;
}
sub _isa_color {
my $color = shift;
return $color =~ /white|black|FFFFFF|000000/i ? 0 : 1;
}
sub level { -1 }
# Need to override this so we have a nice image map for overlayed peaks
sub boxes {
my $self = shift;
my($left,$top,$parent) = @_;
return if $self->glyph_subtype eq 'density'; # No boxes for density plot
my @boxes = $self->SUPER::boxes(@_);
if (my $rects = $self->{peak_cache}) {
push @boxes,[@$_,$parent] foreach @$rects;
}
return wantarray ? @boxes : \@boxes;
}
# Modified and fused functions from wiggle_density.pm and wiggle_xyplot.pm
sub _draw_wigfile {
my $self = shift;
my $feature = shift;
my $wig = shift;
$wig->smoothing($self->get_smoothing);
$wig->window($self->smooth_window);
my ($gd,$left,$top) = @_;
my ($start,$end) = $self->effective_bounds($feature);
if ($self->glyph_subtype eq 'density') {
my ($x1,$y1,$x2,$y2) = $self->bounds($left,$top);
$self->draw_segment($gd,
$start,$end,
$wig,$start,$end,
1,1,
$x1,$y1,$x2,$y2);
$self->Bio::Graphics::Glyph::xyplot::draw_label(@_) if $self->option('label');
$self->draw_description(@_) if $self->option('description');
} else {
my ($start,$end) = $self->effective_bounds($feature);
$self->wig($wig);
my $parts = $self->create_parts_for_dense_feature($wig,$start,$end);
$self->draw_plot($parts,@_);
}
}
sub peaks {
my $self = shift;
return @{$self->{_peaks}} if $self->{_peaks};
my $feature = $self->feature;
my $db = $feature->object_store;
my ($p_type) = eval{$feature->get_tag_values('peak_type')};
unless ($db && $p_type) {
$self->{_peaks} = [];
return;
}
my @peaks = $db->features(-seq_id => $feature->segment->ref,
-start => $self->panel->start,
-end => $self->panel->end,
-type => $p_type);
$self->{_peaks} = \@peaks;
return @{$self->{_peaks}};
}
1;
=head1 NAME
Bio::Graphics::Glyph::vista_plot - The "vista_plot" glyph
=head1 SYNOPSIS
See
L<Bio::Graphics::Glyph>, L<Bio::Graphics::Glyph::wiggle_xyplot> and L<Bio::Graphics::Glyph::heat_map>.
=head1 DESCRIPTION
This glyph draws peak calls (features with discreet boundaries,
i.e. putative transcription sites, over signal graph (wiggle_xyplot)
requires a special load gff file that uses attributes 'wigfile' and 'peak_type'
B<Example:>
2L chip_seq vista 5407 23011573 . . . Name=ChipSeq Exp 1;wigfile=SomeWigFile.wigdb;peak_type=binding_site:exp1
The glyph will draw the wiggle file first, than overlay the peaks (if there are any)
over signal graph. Elsewhere in the GFF3 file, there should be one or more features
of type "binding_site:exp1", e.g.:
2L exp1 binding_site 91934 92005 . . .
Options like 'balloon hover' and 'link' are available to customize
interaction with peaks in detail view.
B<BigWig support:>
Supported bigwig format also requires another attribute to be supplied
in load gff file (fasta) which specifies sequence index file for the
organism in use. The data file should have the 'bw' extension - it is
used to detect the BigWig format by vista_plot
3L chip_seq vista 1 24543530 . . . Name=ChipSeq Exp 2;wigfile=SomeBigWigFile.bw;peak_type=binding_site:exp2;fasta=YourOrganism.fasta
Note that all attributes should be present in load gff, as the code currently does not handle situation when
only some of the attributes are in gff. To omit peak or signal drawing use "" (i.e. peak_type="")
In both cases, the stanza code will look the same (only essential parameters shown):
[VISTA_PLOT]
feature = vista:chip_seq
glyph = vista_plot
label = 1
smoothing = mean
smoothing_window = 10
bump density = 250
autoscale = local
variance_band = 1
max_peak = 255
min_peak = 1
peakwidth = 3
start_color = lightgray
end_color = black
pos_color = blue
neg_color = orange
bgcolor = orange
alpha = 80
fgcolor = black
database = database_with_load_gff_data
box_subparts = 1
bicolor_pivot = min
key = VISTA plot
=head1 OPTIONS
Options are the same as for wiggle_xyplot and heat_map
B<Additional parameters:>
B<alpha>
set transparency for peak area.
B<glyph_subtype>
Display only 'peaks', 'signal', 'density' or 'peaks+signal'.
Aliases for 'peaks+signal' include "both" and "vista".
B<Recommended global settings:>
( run in 2.260 seconds using v1.01-cache-2.11-cpan-437f7b0c052 )