Bio-EnsEMBL
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=head1 LICENSE
See the NOTICE file distributed with this work for additional information
regarding copyright ownership.
Licensed under the Apache License, Version 2.0 (the "License");
you may not use this file except in compliance with the License.
You may obtain a copy of the License at
http://www.apache.org/licenses/LICENSE-2.0
Unless required by applicable law or agreed to in writing, software
distributed under the License is distributed on an "AS IS" BASIS,
WITHOUT WARRANTIES OR CONDITIONS OF ANY KIND, either express or implied.
See the License for the specific language governing permissions and
limitations under the License.
=cut
=head1 CONTACT
Please email comments or questions to the public Ensembl
developers list at <http://lists.ensembl.org/mailman/listinfo/dev>.
Questions may also be sent to the Ensembl help desk at
<http://www.ensembl.org/Help/Contact>.
=cut
=head1 NAME
Bio::EnsEMBL::MicroRNA - A class representing a microRNA product
of a transcript
=head1 DESCRIPTION
A specialisation of Bio::EnsEMBL::RNAProduct describing
MicroRNAs. Mostly takes care of wrapping miRNA-specific RNAProduct
attributes in methods which make them look like ordinary class
members.
=head1 SYNOPSIS
my $miR = Bio::EnsEMBL::MicroRNA->new(
-SEQ_START => 36,
-SEQ_END => 58
);
# Stable-ID setter
$miR->stable_id('ENSS00090210');
# Get start and end position in the precursor transcript
my $start = $miR->start();
my $end = $miR->end();
=cut
package Bio::EnsEMBL::MicroRNA;
$Bio::EnsEMBL::MicroRNA::VERSION = '114.0.0';
use vars qw($AUTOLOAD);
use strict;
use warnings;
use Bio::EnsEMBL::Utils::Exception qw( throw warning );
use Bio::EnsEMBL::Utils::Argument qw( rearrange );
use Bio::EnsEMBL::Utils::Scalar qw( assert_ref wrap_array );
use Scalar::Util qw(weaken);
use Bio::EnsEMBL::RNAProduct;
use parent qw(Bio::EnsEMBL::RNAProduct);
=head2 new
Arg: [-ARM] : which arm of the hairpin precursor this miRNA comes
from. Returns 3 and 5 for 3' and 5', respectively.
Arg [...] : Named arguments to superclass constructor
(see Bio::EnsEMBL::RNAProduct)
Example : my $miR = Bio::EnsEMBL::MicroRNA->new(
-SEQ_START => 36,
-SEQ_END => 58,
-ARM => 3
);
Description: Constructor. Creates a new MicroRNA object
Returntype : Bio::EnsEMBL::MicroRNA
Exceptions : throw if ARM value is out of bounds
Caller : general
Status : In Development
=cut
# perlcritic doesn't know about rearrange(), silence it
sub new { ## no critic (Subroutines::RequireArgUnpacking)
my $caller = shift;
my $class = ref($caller) || $caller;
my $self = $class->SUPER::new(@_);
my ($arm) = rearrange(["ARM"], @_);
if (defined($arm)) {
_validate_arm_value($arm);
}
$self->{'arm'} = $arm;
return $self;
}
=head2 arm
Arg [1] : (optional) int $arm which arm of the hairpin precursor
this miRNA comes from
Example : $mirna_arm = $mirna->arm();
$mirna->arm(3);
Description : Sets or returns the arm of the hairpin this miRNA comes
from. Accepted values are 3 and 5 for 3' and 5',
respectively.
Return type : Integer
Exceptions : throw if setter is passed an incorrect value
or if multiple 'mirna_arm' attributes exist.
Caller : General
Status : Stable
=cut
sub arm {
my ($self, $arm) = @_;
if (defined $arm) {
_validate_arm_value($arm);
$self->{'arm'} = $arm;
} elsif (!defined($self->{'arm'})) {
my $arm_attrs = $self->get_all_Attributes('mirna_arm');
my $n_arms = scalar @{$arm_attrs};
if ($n_arms > 0) {
if ($n_arms > 1) {
throw("MicroRNA " . $self->display_id() .
" has multiple arm attributes");
}
$self->{'arm'} = $arm_attrs->[0]->value();
}
}
return $self->{'arm'};
}
=head2 summary_as_hash
Example : $mirna_summary = $mirna->summary_as_hash();
Description : Retrieves a textual summary of this MicroRNA.
Built on top of generic implementation in RNAProduct.
Returns : hashref of arrays of descriptive strings
Status : Intended for internal use
=cut
sub summary_as_hash {
my $self = shift;
my $summary = SUPER::summary_as_hash();
$summary->{'arm'} = $self->arm();
return $summary;
}
=head2 _validate_arm_value
Arg [1] : int $arm which arm of the hairpin precursor this miRNA
comes from
Description: PRIVATE validates if its argument has one of the accepted
values for specifying the miRNA hairpin arm.
Returntype : none
Exceptions : throw if the argument is out of bounds
Caller : internal
Status : Stable
=cut
sub _validate_arm_value {
my ($arm) = @_;
if (($arm != 3) && ($arm != 5)) {
throw("'$arm' is not a valid miRNA hairpin-arm specification");
}
return;
}
1;
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