App-Mimosa
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lib/App/Mimosa/Job.pm view on Meta::CPAN
package App::Mimosa::Job;
use Moose;
use namespace::autoclean;
use autodie ':all';
use Bio::SeqIO;
use Moose::Util::TypeConstraints;
use Bio::BLAST::Database;
use File::Spec::Functions;
use IPC::Run qw/timeout/;
# Good breakdown of commandline flags
# http://www.molbiol.ox.ac.uk/analysis_tools/BLAST/BLAST_blastall.shtml
subtype 'Program'
=> as Str
=> where {
/^(blastn|tblastx|tblastn)$/;
};
subtype 'SubstitutionMatrix'
=> as Str
=> where {
/^(BLOSUM|PAM)\d\d$/;
};
has program => (
isa => 'Program',
is => 'rw',
default => 'blastn',
);
has input_file => (
isa => 'Str',
is => 'rw',
);
has context => (
is => 'rw',
);
has output_file => (
isa => 'Str',
is => 'rw',
);
has evalue => (
isa => 'Num',
is => 'rw',
default => 0.01,
);
has maxhits => (
isa => 'Int',
is => 'rw',
default => 100,
);
has matrix => (
isa => 'SubstitutionMatrix',
is => 'rw',
default => 'BLOSUM62',
);
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