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lib/App/Anchr/Command/break.pm  view on Meta::CPAN

package App::Anchr::Command::break;
use strict;
use warnings;
use autodie;

use App::Anchr -command;
use App::Anchr::Common;

use constant abstract => "break long reads by anthors";

sub opt_spec {
    return (
        [ "outfile|o=s", "output filename, [stdout] for screen", ],
        [ 'range|r=s',    'ranges of anchors',            { required => 1 }, ],
        [ 'border=i',     'length of borders in anchors', { default  => 100 }, ],
        [ 'power=f',      'multiplying power',            { default  => 3 }, ],
        [ "len|l=i",      "minimal length of overlaps",   { default  => 1000 }, ],
        [ "idt|i=f",      "minimal identity of overlaps", { default  => 0.85 }, ],
        [ "parallel|p=i", "number of threads",            { default  => 4 }, ],
        [ "verbose|v",    "verbose mode", ],
        { show_defaults => 1, }
    );
}

sub usage_desc {
    return "anchr break [options] <dazz DB> <ovlp file>";
}

sub description {
    my $desc;
    $desc .= ucfirst(abstract) . ".\n";
    $desc .= "\tThis command relies on an existing dazz db.\n";
    return $desc;
}

sub validate_args {
    my ( $self, $opt, $args ) = @_;

    if ( @{$args} != 2 ) {
        my $message = "This command need one or more input files.\n\tIt found";
        $message .= sprintf " [%s]", $_ for @{$args};
        $message .= ".\n";
        $self->usage_error($message);
    }
    for ( @{$args} ) {
        if ( !Path::Tiny::path($_)->is_file ) {
            $self->usage_error("The input file [$_] doesn't exist.");
        }
    }

    if ( !AlignDB::IntSpan->valid( $opt->{range} ) ) {
        $self->usage_error("Invalid --range [$opt->{range}]\n");
    }

    if ( !exists $opt->{outfile} ) {
        $opt->{outfile} = Path::Tiny::path( $args->[0] )->absolute . ".breaks.fasta";
    }
}

sub execute {
    my ( $self, $opt, $args ) = @_;

    # absolute paths before we chdir to $out_dir
    my $fn_dazz = Path::Tiny::path( $args->[0] )->absolute->stringify;
    my $fn_ovlp = Path::Tiny::path( $args->[1] )->absolute->stringify;

    if ( lc $opt->{outfile} ne "stdout" ) {
        $opt->{outfile} = Path::Tiny::path( $opt->{outfile} )->absolute->stringify;
    }

    # record cwd, we'll return there
    my $cwd     = Path::Tiny->cwd;
    my $tempdir = Path::Tiny->tempdir("anchr_break_XXXXXXXX");
    chdir $tempdir;

    #@type AlignDB::IntSpan
    my $anchor_range = AlignDB::IntSpan->new->add_runlist( $opt->{range} );

    #----------------------------#