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samples
(
Figure
5
)
.
Although
the
number
of
AOB
calculated
using
the
16S
rDNA
assay
were
about
2-fold
the
values
calculated
using
the
amoA
assays
,
the
assays
were
significantly
correlated
with
a
Pearsons
coefficient
of
0.901
.
Ammonia-oxidizing
activity
per
cell
‚
hour
was
calculated
from
the
AOB
cell
number
and
WWTP
plant
data
using
the
following
formula
:
activity
)
+
(
NH
+
-
NH
+
-
NH4OUT
)
×
Q
4IN
4UP
AOB
NH4
+
IN
is
the
average
ammonia
concentration
(
measured
in
mg
nitrogen
/
L
)
in
the
secondary
influent
into
the
WWTP
reactor.
NH4
+
OUT
is
the
average
ammonia
concentration
in
the
plant
effluent
.
NH4
+
UP
is
used