BioPerl
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Bio/SeqIO/interpro.pm view on Meta::CPAN
with code and data examples if at all possible.
=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
of the bugs and their resolution. Bug reports can be submitted via
the web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Jared Fox
Email jaredfox@ucla.edu
=head1 CONTRIBUTORS
Allen Day allenday@ucla.edu
=head1 APPENDIX
The rest of the documentation details each of the object methods.
Internal methods are usually preceded with a _
=cut
# Let the code begin...
package Bio::SeqIO::interpro;
use strict;
use Bio::SeqFeature::Generic;
use XML::DOM;
use XML::DOM::XPath;
use Bio::Seq::SeqFactory;
use Bio::Annotation::Collection;
use Bio::Annotation::DBLink;
use base qw(Bio::SeqIO);
my $idcounter = {}; # Used to generate unique id values
my $nvtoken = ": "; # The token used if a name/value pair has to be stuffed
# into a single line
=head2 next_seq
Title : next_seq
Usage : my $seqobj = $stream->next_seq
Function: Retrieves the next sequence from a SeqIO::interpro stream.
Returns : A Bio::Seq::RichSeq object
Args :
=cut
sub next_seq {
my $self = shift;
my ($desc);
my $bioSeq = $self->_sequence_factory->create(-verbose =>$self->verbose());
my $zinc = "(\"zincins\")";
my $wing = "\"Winged helix\"";
my $finger = "\"zinc finger\"";
my $xml_fragment = undef;
while(my $line = $self->_readline()){
my $where = index($line, $zinc);
my $wherefinger = index($line, $finger);
my $finishedline = $line;
my $wingwhere = index($line, $wing);
# the interpro XML is not fully formed, so we need to convert the
# extra double quotes and ampersands into appropriate XML character codes
if($where > 0){
my @linearray = split /$zinc/, $line;
$finishedline = join ""zincins"", $linearray[0], $linearray[2];
}
if(index($line, "&") > 0){
my @linearray = split /&/, $line;
$finishedline = join "&", $linearray[0], $linearray[1];
}
if($wingwhere > 0){
my @linearray = split /$wing/, $line;
$finishedline = join ""Winged helix"", $linearray[0], $linearray[1];
}
$xml_fragment .= $finishedline;
last if $finishedline =~ m!</protein>!;
}
# Match <protein> but not other similar elements like <protein-matches>
return unless $xml_fragment =~ /<protein[\s>]/;
$self->_parse_xml($xml_fragment);
my $dom = $self->_dom;
my ($protein_node) = $dom->findnodes('/protein');
my @interproNodes = $protein_node->findnodes('/protein/interpro');
my @DBNodes = $protein_node->findnodes('/protein/interpro/match');
for(my $interpn=0; $interpn<scalar(@interproNodes); $interpn++){
my $ipnlevel = join "", "/protein/interpro[", $interpn + 1, "]";
my @matchNodes = $protein_node->findnodes($ipnlevel);
for(my $match=0; $match<scalar(@matchNodes); $match++){
my $matlevel = join "", "/protein/interpro[", $interpn+1, "]/match[",
$match+1, "]/location";
my @locNodes = $protein_node->findnodes($matlevel);
my $class_level = join "", "/protein/interpro[",$interpn+1, "]/classification";
my @goNodes = $protein_node->findnodes($class_level);
my @seqFeatures = map { Bio::SeqFeature::Generic->new(
-start => $_->getAttribute('start'),
-end => $_->getAttribute('end'),
-score => $_->getAttribute('score'),
-source_tag => 'IPRscan',
-primary_tag => 'region',
-display_name => $interproNodes[$interpn]->getAttribute('name'),
-seq_id => $protein_node->getAttribute('id') ),
} @locNodes;
foreach my $seqFeature (@seqFeatures){
$bioSeq->add_SeqFeature($seqFeature);
my $annotation1 = Bio::Annotation::DBLink->new;
$annotation1->database($matchNodes[$match]->getAttribute('dbname'));
$annotation1->primary_id($matchNodes[$match]->getAttribute('id'));
$annotation1->comment($matchNodes[$match]->getAttribute('name'));
$seqFeature->annotation->add_Annotation('dblink',$annotation1);
my $annotation2 = Bio::Annotation::DBLink->new;
$annotation2->database('INTERPRO');
$annotation2->primary_id($interproNodes[$interpn]->getAttribute('id'));
$annotation2->comment($interproNodes[$interpn]->getAttribute('name'));
$seqFeature->annotation->add_Annotation('dblink',$annotation2);
# Bug 1908 (enhancement)
my $annotation3 = Bio::Annotation::DBLink->new;
$annotation3->database($DBNodes[$interpn]->getAttribute('dbname'));
$annotation3->primary_id($DBNodes[$interpn]->getAttribute('id'));
$annotation3->comment($DBNodes[$interpn]->getAttribute('name'));
$seqFeature->annotation->add_Annotation('dblink',$annotation3);
# need to put in the go annotation here!
foreach my $g (@goNodes)
{
my $goid = $g->getAttribute('id');
my $go_annotation = Bio::Annotation::DBLink->new;
$go_annotation->database('GO');
$go_annotation->primary_id($goid);
$go_annotation->comment($goid);
$seqFeature->annotation->add_Annotation('dblink', $go_annotation);
}
}
}
}
my $accession = $protein_node->getAttribute('id');
my $displayname = $protein_node->getAttribute('name');
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