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=head2 Reporting Bugs
Report bugs to the Bioperl bug tracking system to help us keep track
the bugs and their resolution. Bug reports can be submitted via the
web:
https://github.com/bioperl/bioperl-live/issues
=head1 AUTHOR - Robson Francisco de Souza
Email: rfsouza@citri.iq.usp.br
=head1 APPENDIX
The rest of the documentation details each of the object
methods. Internal methods are usually preceded with a _
=cut
#
# Now, let's code!
package Bio::Assembly::ScaffoldI;
use strict;
use Carp;
# Inheritance
use base qw(Bio::Root::RootI);
#
# Methods
=head1 Accessing general assembly data
=cut
=head2 get_nof_contigs
Title : get_nof_contigs
Usage : $assembly->get_nof_contigs()
Function: Get the number of contigs included in the assembly
Returns : integer
Args : none
=cut
sub get_nof_contigs {
my $self = shift;
$self->throw_not_implemented();
}
=head2 get_nof_singlets
Title : get_nof_singlets
Usage : $assembly->get_nof_singlets()
Function: Get the number of singlets included in the assembly
Returns : integer
Args : none
=cut
sub get_nof_singlets {
my $self = shift;
$self->throw_not_implemented();
}
=head2 get_contig_ids
Title : get_contig_ids
Usage : $assembly->get_contig_ids()
Function: Access list of contig IDs from assembly
Returns : an array if there are any contigs in the assembly.
undef otherwise
Args : an array of contig IDs
=cut
sub get_contig_ids {
my $self = shift;
$self->throw_not_implemented();
}
=head2 get_singlet_ids
Title : get_singlet_ids
Usage : $assembly->get_singlet_ids()
Function: Access list of singlet IDs from assembly
Returns : an array if there are any singlets in the assembly.
undef otherwise
Args : an array of singlet IDs
=cut
sub get_singlet_ids {
my $self = shift;
$self->throw_not_implemented();
}
=head2 get_contig_by_id
Title : get_contig_by_id
Usage : $assembly->get_contig_by_id($id)
Function: Get a reference for a contig from the assembly
Returns : a Bio::Assembly::Contig object or undef
Args : [string] contig unique identifier (ID)
=cut
sub get_contig_by_id {
my $self = shift;
$self->throw_not_implemented();
}
=head2 get_singlet_by_id
Title : get_singlet_by_id
Usage : $assembly->get_singlet_by_id()
Function: Get a reference for a singlet from the assembly
Returns : Bio::Assembly::Singlet object or undef
Args : [string] a singlet ID
=cut
sub get_singlet_by_id {
my $self = shift;
$self->throw_not_implemented();
}
=head1 Modifier methods
Implementation of these methods is optional in the sense that
read-only implementations may not have these. If an object implements
one of them, it should however implement all.
=cut
=head2 add_contig
Title : add_contig
Usage : $assembly->add_contig($contig)
Function: Add another contig to the Bio::Assembly::ScaffoldI object
Returns : 1 on success, 0 otherwise
Args : a Bio::Assembly:Contig object
See Bio::Assembly::Contig for more information
=cut
#---------------------
sub add_contig {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 add_singlet
Title : add_singlet
Usage : $assembly->add_singlet($seq)
Function: Add another singlet to the Bio::Assembly::ScaffoldI object
Returns : 1 on success, 0 otherwise
Args : a Bio::Assembly::Singlet object
=cut
#---------------------
sub add_singlet {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 remove_contigs
Title : remove_contigs
Usage : $assembly->remove_contigs(1..4)
Function: Remove contig from assembly object
Returns : a Bio::Assembly::Contig object
Args : a list of contig IDs
See function get_contig_ids() above
=cut
#---------------------
sub remove_contigs {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 remove_singlets
Title : remove_singlets
Usage : $assembly->remove_singlets(1..4)
Function: Remove singlets from assembly object
Returns : an array of Bio::Assembly::Singlet objects
Args : an array of singlet IDs
See function get_singlet_ids() above
=cut
#---------------------
sub remove_singlets {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head1 Contig and singlet selection methos
=cut
=head2 select_contigs
Title : select_contig
Usage : $assembly->select_contig
Function: Selects an array of contigs from the assembly
Returns : an array of Bio::Assembly::Contig objects
Args : an array of contig ids
See function get_contig_ids() above
=cut
#---------------------
sub select_contigs {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 select_singlets
Title : select_singlets
Usage : $assembly->select_singlets(@list)
Function: Selects an array of singlets from the assembly
Returns : an array of Bio::Assembly::Singlet objects
Args : an array of singlet ids
See function get_singlet_ids() above
=cut
#---------------------
sub select_singlets {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 all_contigs
Title : all_contigs
Usage : my @contigs = $assembly->all_contigs
Function: Returns a list of all contigs in this assembly.
Contigs are both clusters and alignments of one
or more reads, with an associated consensus
sequence.
Returns : array of Bio::Assembly::Contig
Args : none
=cut
#---------------------
sub all_contigs {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
=head2 all_singlets
Title : all_singlets
Usage : my @singlets = $assembly->all_singlets
Function: Returns a list of all singlets in this assembly.
Singlets are isolated reads, without non-vector
matches to any other read in the assembly.
Returns : array of Bio::Assembly::Singlet objects
Args : none
=cut
#---------------------
sub all_singlets {
#---------------------
my ($self) = @_;
$self->throw_not_implemented();
}
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