AcePerl
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Ace/Graphics/Glyph/segments.pm view on Meta::CPAN
if ($stranded
&& $segments[$i]->can('target')
&& ($target = $segments[$i]->target)
&& $target->can('start')) {
$strand = $target->start < $target->end ? 1 : -1;
}
# probably unnecessary, but we do it out of paranaoia
($start,$stop) = ($stop,$start) if $start > $stop;
push @boxes,[$start,$stop,$strand];
if (my $next_segment = $segments[$i+1]) {
my ($next_start,$next_stop) = ($left + $self->map_pt($next_segment->start),
$left + $self->map_pt($next_segment->end));
# probably unnecessary, but we do it out of paranaoia
($next_start,$next_stop) = ($next_stop,$next_start) if $next_start > $next_stop;
# fudge boxes that are within two pixels of each other
if ($next_start - $stop < 2) {
$boxes[-1][1] = $next_start;
}
push @skips,[$stop+1,$next_start-1];
}
}
my $fg = $self->fgcolor;
my $fill = $self->fillcolor;
my $center = ($y1 + $y2)/2;
# each segment becomes a box
for my $e (@boxes) {
my @rect = ($e->[0],$y1,$e->[1],$y2);
if ($e->[2] == 0 || !$stranded) {
$self->filled_box($gd,@rect);
} else {
# $self->filled_arrow($gd,1,@rect);
$self->oriented_box($gd,$e->[2],@rect);
}
}
# each skip becomes a simple line
for my $i (@skips) {
next unless $i->[1] - $i->[0] >= 1;
$gd->line($i->[0],$center,$i->[1],$center,$gray);
}
# draw label
$self->draw_label($gd,@_) if $self->option('label');
}
sub oriented_box {
my $self = shift;
my $gd = shift;
my $orientation = shift;
my ($x1,$y1,$x2,$y2) = @_;
$self->filled_box($gd,@_);
return unless $x2 - $x1 >= 4;
$BRUSHES{$orientation} ||= $self->make_brush($orientation);
my $top = int(1.5 + $y1 + ($y2 - $y1 - ($BRUSHES{$orientation}->getBounds)[1])/2);
$gd->setBrush($BRUSHES{$orientation});
$gd->setStyle(0,0,0,1);
$gd->line($x1+2,$top,$x2-2,$top,gdStyledBrushed);
}
sub make_brush {
my $self = shift;
my $orientation = shift;
my $brush = GD::Image->new(3,3);
my $bgcolor = $brush->colorAllocate(255,255,255); #white
$brush->transparent($bgcolor);
my $fgcolor = $brush->colorAllocate($self->factory->panel->rgb($self->fgcolor));
if ($orientation > 0) {
$brush->setPixel(0,0,$fgcolor);
$brush->setPixel(1,1,$fgcolor);
$brush->setPixel(0,2,$fgcolor);
} else {
$brush->setPixel(1,0,$fgcolor);
$brush->setPixel(0,1,$fgcolor);
$brush->setPixel(1,2,$fgcolor);
}
$brush;
}
sub description {
my $self = shift;
$self->feature->info;
}
1;
__END__
=head1 NAME
Ace::Graphics::Glyph::segments - The "discontinuous segments" glyph
=head1 SYNOPSIS
See L<Ace::Graphics::Panel> and L<Ace::Graphics::Glyph>.
=head1 DESCRIPTION
This glyph draws a sequence feature that consists of multiple
discontinuous segments, such as the exons on a transcript or a gapped
alignment. The representation is a series of filled rectangles
connected by line segments.
The features passed to it must either respond to the
Bio::SequenceFeatureI-style subSeqFeatures() method, or the
AcePerl/Das-style segments() or merged_segments() methods.
=head2 OPTIONS
In addition to the common options, this glyph recognizes the
b<-stranded> argument. If b<-stranded> is true and the feature is an
alignment (has the target() method) then the glyph will draw little
arrows in the segment boxes to indicate the direction of the
alignment.
=head1 BUGS
Please report them.
=head1 SEE ALSO
L<Ace::Sequence>, L<Ace::Sequence::Feature>, L<Ace::Graphics::Panel>,
L<Ace::Graphics::Track>,
L<Ace::Graphics::Glyph::anchored_arrow>,
L<Ace::Graphics::Glyph::arrow>,
L<Ace::Graphics::Glyph::box>,
L<Ace::Graphics::Glyph::primers>,
L<Ace::Graphics::Glyph::segments>,
L<Ace::Graphics::Glyph::toomany>,
L<Ace::Graphics::Glyph::transcript>,
=head1 AUTHOR
Lincoln Stein <lstein@cshl.org>.
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