ABI

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ABI.pm  view on Meta::CPAN

  my $seq = $abi->get_sequence(); # To get the sequence
  my @trace_a = $abi->get_trace("A"); # Get the raw traces for "A"
  my @trace_g = $abi->get_trace("G"); # Get the raw traces for "G"
  my @base_calls = $abi->get_base_calls(); # Get the base calls

=head1 DESCRIPTION

An ABI chromatogram file is in binary format. It contain several 
information only some of which is required for normal use. This
module only gives access to the most used information stored in
ABI file. All the accesses are read only.

If you have edited the file using a trace editor, then you can use the corresponding 
method to access the edited sequence and base calls.



=head1 CONSTRUCTOR

=head2 new()

  Usage : $abi = ABI->new(-file=>"filename");
          $abi = ABI->new("filename"); # same thing

=cut

sub new {
	my $class = shift;
	my $self  = {};
	bless $self, ref($class) || $class;
	$self->_init(@_);

	#print "****", $self->{_mac_header}, "\n";
	return $self;
}

sub _init {
	my ( $self, @args ) = @_;
	my ($file) = $self->_rearrange( ["FILE"], @args );
	if ( !defined($file) ) {
		croak "Can't open the input file\n";
	} else {
		$self->set_file_handle($file);
	}
	$self->{_sequence}             = "";
	$self->{_sequence_corrected}   = "";
	$self->{_sample}               = "";
	$self->{A}                     = [];
	$self->{T}                     = [];
	$self->{G}                     = [];
	$self->{C}                     = [];
	$self->{_basecalls}            = [];
	$self->{_basecalls_corrected}  = [];
	$self->{_trace_length}         = 0;
	$self->{_seq_length}           = 0;
	$self->{_seq_length_corrected} = 0;
	$self->{_abs_index}            = 26;
	$self->{_index}                = undef;
	$self->{PLOC1}                 = undef;
	$self->{PLOC}                  = undef;
	$self->{_a_start}              = undef;
	$self->{_g_start}              = undef;
	$self->{_c_start}              = undef;
	$self->{_t_start}              = undef;
	$self->{DATA9}                 = undef;
	$self->{DATA10}                = undef;
	$self->{DATA11}                = undef;
	$self->{DATA12}                = undef;
	$self->{PBAS1}                 = undef;
	$self->{PBAS2}                 = undef;
	$self->{FWO}                   = undef;
	$self->{_mac_header}           = 0;
	$self->{_maximum_trace}        = 0;

	if ( $self->_is_abi() ) {

		#print "ABI FILE\n";
		$self->_set_index();
		$self->_set_base_calls();
		$self->_set_corrected_base_calls();
		$self->_set_seq();
		$self->_set_corrected_seq();
		$self->_set_traces();
		$self->_set_max_trace();
		$self->_set_sample_name();
		close( $self->{_fh} );
	}
	return $self;
}

sub set_file_handle {
	my $self = shift;
	my $path = shift;
	my $fh   = IO::File->new();
	if ( $fh->open("< $path") ) {
		binmode($fh);
		$self->{_fh} = $fh;
	} else {
		croak "Could not open $path in ABITrace::set_file_handle\n";
	}
}

sub _rearrange {
	my ( $self, $order, @param ) = @_;
	return unless @param;
	return @param unless ( defined( $param[0] ) && $param[0] =~ /^-/ );
	for ( my $i = 0 ; $i < @param ; $i += 2 ) {
		$param[$i] =~ s/^\-//;
		$param[$i] =~ tr/a-z/A-Z/;
	}

	# Now we'll convert the @params variable into an associative array.
	local ($^W) = 0;    # prevent "odd number of elements" warning with -w.
	my (%param) = @param;
	my (@return_array);

	# What we intend to do is loop through the @{$order} variable,
	# and for each value, we use that as a key into our associative
	# array, pushing the value at that key onto our return array.
	my ($key);
	foreach $key ( @{$order} ) {
		my ($value) = $param{$key};
		delete $param{$key};
		push( @return_array, $value );



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