ABI
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sub _init {
my ( $self, @args ) = @_;
my ($file) = $self->_rearrange( ["FILE"], @args );
if ( !defined($file) ) {
croak "Can't open the input file\n";
} else {
$self->set_file_handle($file);
}
$self->{_sequence} = "";
$self->{_sequence_corrected} = "";
$self->{_sample} = "";
$self->{A} = [];
$self->{T} = [];
$self->{G} = [];
$self->{C} = [];
$self->{_basecalls} = [];
$self->{_basecalls_corrected} = [];
$self->{_trace_length} = 0;
$self->{_seq_length} = 0;
$self->{_seq_length_corrected} = 0;
$self->{_abs_index} = 26;
$self->{_index} = undef;
$self->{PLOC1} = undef;
$self->{PLOC} = undef;
$self->{_a_start} = undef;
$self->{_g_start} = undef;
$self->{_c_start} = undef;
$self->{_t_start} = undef;
$self->{DATA9} = undef;
$self->{DATA10} = undef;
$self->{DATA11} = undef;
$self->{DATA12} = undef;
$self->{PBAS1} = undef;
$self->{PBAS2} = undef;
$self->{FWO} = undef;
$self->{_mac_header} = 0;
$self->{_maximum_trace} = 0;
if ( $self->_is_abi() ) {
#print "ABI FILE\n";
$self->_set_index();
$self->_set_base_calls();
$self->_set_corrected_base_calls();
$self->_set_seq();
$self->_set_corrected_seq();
$self->_set_traces();
$self->_set_max_trace();
$self->_set_sample_name();
close( $self->{_fh} );
}
return $self;
}
sub set_file_handle {
my $self = shift;
my $path = shift;
my $fh = IO::File->new();
if ( $fh->open("< $path") ) {
binmode($fh);
$self->{_fh} = $fh;
} else {
croak "Could not open $path in ABITrace::set_file_handle\n";
}
}
sub _rearrange {
my ( $self, $order, @param ) = @_;
return unless @param;
return @param unless ( defined( $param[0] ) && $param[0] =~ /^-/ );
for ( my $i = 0 ; $i < @param ; $i += 2 ) {
$param[$i] =~ s/^\-//;
$param[$i] =~ tr/a-z/A-Z/;
}
# Now we'll convert the @params variable into an associative array.
local ($^W) = 0; # prevent "odd number of elements" warning with -w.
my (%param) = @param;
my (@return_array);
# What we intend to do is loop through the @{$order} variable,
# and for each value, we use that as a key into our associative
# array, pushing the value at that key onto our return array.
my ($key);
foreach $key ( @{$order} ) {
my ($value) = $param{$key};
delete $param{$key};
push( @return_array, $value );
}
# print "\n_rearrange() after processing:\n";
# my $i; for ($i=0;$i<@return_array;$i++) { printf "%20s => %s\n", ${$order}[$i], $return_array[$i]; } <STDIN>;
return (@return_array);
}
sub _is_abi {
my $self = shift;
my $fh = $self->{"_fh"};
my $buf;
seek( $fh, 0, 0 );
read( $fh, $buf, 3 );
#my $a = unpack("n*", $buf);
if ( $buf eq "ABI" ) {
return 1;
} else {
seek( $fh, 128, 0 );
read( $fh, $buf, 3 );
if ( $buf eq "ABI" ) {
$self->_set_mac_header();
return 1;
} else {
return 0;
}
}
}
sub _set_mac_header {
my $self = shift;
$self->{_mac_header} = 128;
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