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lib/Bio/Tools/Primer3Redux/Primer.pm  view on Meta::CPAN

        $self->remove_tag('tm') if $self->has_tag('tm');
        $self->add_tag_value('tm', $tm);
    }
    $self->has_tag('tm') ? return ($self->get_tag_values('tm'))[0] : return;
}


sub gc_content {
    my ($self, $gc) = @_;
    if (defined $gc) {
        $self->remove_tag('gc_percent') if $self->has_tag('gc_percent');
        $self->add_tag_value('gc_percent', $gc);
    }
    $self->has_tag('gc_percent') ? return ($self->get_tag_values('gc_percent'))[0] : return;
}


sub run_description {
    my ($self, $desc) = @_;
    if (defined $desc) {
        $self->remove_tag('explain') if $self->has_tag('explain');
        $self->add_tag_value('explain', $desc);
    }
    $self->has_tag('explain') ? return ($self->get_tag_values('explain'))[0] : return;

lib/Bio/Tools/Primer3Redux/Primer.pm  view on Meta::CPAN

 Usage    : $obj->melting_temp
 Function : returns the Tm calculated for the primer via Primer3
 Returns  : float
 Args     : optional Tm (possibly calculated via other means)

=head2 gc_content

 Title    : gc
 Usage    : $obj->gc
 Function : returns the GC content calculated for the primer via Primer3
 Returns  : float (percent)
 Args     : optional GC content (possibly calculated via other means)

=head2 run_description

 Title    : run_description
 Usage    : $obj->run_description
 Function : returns the run description for this primer (via Primer3)
 Returns  : string
 Args     : optional description