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<protocol xml:space="preserve"
  title="TIGR: Hybridization"
  type="hybridization">The goal in any hybridization is to obtain high
 specificity while minimizing background. We have
 developed protocols that give reproducible,
 high-quality hybridization results while maximizing
 the measured fluorescence from the array. 
 Aminosilane coated slides bind DNA with high
 efficiency. Prior to hybridization, the free amine groups
 on the slide must be blocked or inactivated, otherwise
 nonspecific binding of labeled cDNA to the slide can
 deplete the probe and produce high background.
 Although the slides can be blocked chemically, we have
 found a simple prehybridization in a solution
 containing 1% bovine serum albumin to be extremely
 effective in eliminating nonspecific binding of the probe
 to the slide. 
 Prehybridization has the additional advantage of
 washing unbound DNA from the slide prior to the
 addition of the probe. Any DNA that washes from the
 surface during hybridization competes with DNA
 bound to the slide. As the kinetics of solution
 hybridization is much more favorable than surface